Taken together, iNOS is upregulated after transplantation of FG,

Taken together, iNOS is upregulated after transplantation of FG, leading to excessive RNS formation, JNK activation, mitochondrial dysfunction, and severe graft injury. The iNOS inhibitor 1400W could be an effective therapy for primary nonfunction of fatty liver grafts. (C) 2012

Elsevier Inc. All rights reserved.”
“Stable graft function in the absence of maintenance immunosuppressive therapy (IS) is observed in some kidney recipients and in approximately 20% of liver recipients. Identification of these “operationally tolerant” recipients would constitute a major achievement in that it would allow for the widespread application of IS minimization strategies in the clinic. The use of gene expression profiling has provided promising results in the characterization Compound C of tolerant transplant recipients. We review here the available information on the use of

transcriptional biomarkers to identify transplantation tolerance in the clinic and discuss some of the limitations of such strategy.”
“Objective. This study’s aim was to assess the effect of highly active antiretroviral drugs (HAART) on benign lymphoepithelial cysts (BLEC) of the parotid and ranulas.\n\nStudy design. The records of 10 HIV-positive patients who presented with BLEC were reviewed, and 14 HIV-positive patients who presented with ranulas were prospectively enrolled. Patients in both groups GSK690693 received the same combination of HAART LY2157299 datasheet and were clinically evaluated for the first 3 months. Patients with ranulas were followed for an additional 3 months. A clinical reduction in the size of the lesions was considered to be a positive outcome.\n\nResults. All parotid glands with BLEC resolved within 3 months. No positive results were observed in ranulas during the first 3

months. However, some of the ranulas displayed a positive result after the initial 3 months of HAART.\n\nConclusions. In contrast to BLEC, ranulas in HIV-positive patients seem to present a mixed and delayed response to HAART. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;111:205-210)”
“Background Hard-to-reach population subgroups are typically investigated using convenience sampling, which may give biased estimates. Combining information from such surveys, a probability survey and clinic surveillance, can potentially minimize the bias. We developed a methodology to estimate the prevalence of undiagnosed HIV infection among men who have sex with men (MSM) in England and Wales aged 16-44 years in 2003, making fuller use of the available data than earlier work.\n\nMethods We performed a synthesis of three data sources: genitourinary medicine clinic surveillance (11 380 tests), a venue-based convenience survey including anonymous HIV testing (3702 MSM) and a general population sexual behaviour survey (134 MSM).

“A Thoroughbred gelding in North America

“A Thoroughbred gelding in North America Liproxstatin-1 concentration was evaluated for Actinobacillus peritonitis on three different occasions over a 4-year period. At each presentation, peritoneal fluid had an elevated nucleated cell count (220,000-550,000 cells/mu L) characterised by non-degenerate neutrophils, no visible bacteria, an elevated total protein (4.6-5.5 g/dL) and bacterial culture yielding Actinobacillus spp. Actinobacillus peritonitis appears

to be a regional disease occurring in Australia and less commonly in New Zealand and North America. Recurrence, other than incomplete resolution, has not been previously reported. This case highlights the classical presentation, response to therapy and excellent prognosis despite the alarmingly abnormal peritoneal

fluid characteristic of Actinobacillus peritonitis and questions the role of parasite migration in the pathogenesis. Finally, this case is remarkable HKI-272 research buy because Actinobacillus peritonitis was recurrent over several years in an otherwise normal horse.”
“We present a methodology for implementing discrete-time signal processing operations, such as filtering, with molecular reactions. The reactions produce time-varying output quantities of molecules as a function of time-varying input quantities according to a functional specification. This computation is robust and independent of the reaction rates, provided that the selleck screening library rate constants fall within coarse categories. We describe two approaches: one entails synchronization with a clock signal, implemented through sustained chemical oscillations; the other is self-timed or asynchronous. We illustrate the methodology by synthesizing a simple moving-average filter, a biquad filter, and a Fast Fourier Transform (FFT). Abstract molecular reactions for these filters and transforms are translated into DNA strand displacement reactions. The computation is validated through mass-action simulations of the DNA kinetics. Although

a proof of concept for the time being, molecular filters and transforms have potential applications in fields such as biochemical sensing and drug delivery.”
“Purpose: This study aims to identify and compare the relevance of barriers that nurses in nursing homes experience in medication management in Belgium.\n\nDesign: The mixed-method study started with an expert meeting in November 2008 and was followed by a cross-sectional survey in February-March 2009, questioning 246 nurses and 270 nurse assistants in 20 nursing homes.\n\nMethods: Twelve nurses represented nursing homes in an expert meeting and listed all barriers that might cause suboptimal medication management.

002 for all sleep parameters) After adjusting for multiple poten

002 for all sleep parameters). After adjusting for multiple potential confounders, self-reported LDC000067 solubility dmso poor sleep

quality (Pittsburgh Sleep Quality Index > 5, multivariable odds ratio (MOR)=1.28, 95% confidence interval (CI)=1.09-1.50), sleep efficiency less than 70% (MOR=1.37, 95% CI=1.12-1.67), sleep latency of 60 minutes or longer (MOR=1.42, 95% CI=1.10-1.82), and sleep-disordered breathing (respiratory disturbance index >= 15, MOR=1.38, 95% CI=1.15-1.65) were each independently associated with higher odds of greater frailty status.\n\nCONCLUSION\n\nSleep disturbances, including poor self-reported sleep quality, lower sleep efficiency, prolonged sleep latency, and sleep-disordered breathing, are independently associated with greater evidence of frailty.”
“Background: A 13-valent pneumococcal conjugate vaccine (PCV13) containing 6 additional serotypes not included in the 7-valent PCV has been developed to broaden protection against Streptococcus pneumoniae, which is responsible for over 500,000 deaths annually worldwide in children <5 years of age.

This study in Japanese infants evaluated the immunogenicity and safety of PCV13 given subcutaneously, the standard route for infant vaccination Dinaciclib clinical trial in Japan.\n\nMethods: This phase 3, single-arm, open-label study was conducted at 25 sites. Subjects received PCV13 as a 3-dose infant series and a toddler dose. Parents/legal guardians recorded local reactions and systemic events after each vaccination. The proportion of subjects with serotype-specific antipneumococcal polysaccharide immunoglobulin (Ig)G antibody concentrations >= 0.35 mu g/mL was calculated before and 1 month after the infant series and toddler dose.\n\nResults: A total of 193 subjects enrolled. The proportion of subjects achieving pneumococcal IgG antibody concentrations >= 0.35 mu g/mL was >= 97.2% for all 13 pneumococcal serotypes 1 month after the infant series and 98.9-100% after the toddler dose. IgG geometric mean concentrations were

2.57-14.69 mu g/mL after the infant series and 2.06-16.33 mu g/mL after the toddler dose. IgG geometric mean concentrations increased from pre- to posttoddler dose by >= 2.8-fold, demonstrating a booster effect. Local selleck chemical reactions and fever were generally mild or moderate in severity.\n\nConclusions: PCV13 was immunogenic for all serotypes and had a favorable safety profile when administered subcutaneously to Japanese infants. PCV13 should offer broader serotype protection than 7-valent PCV in preventing pneumococcal disease in Japanese children.”
“Addition of 0.5% onion was effective in reducing lipid oxidation in irradiated cooked ground beef after 7 day storage. Addition of garlic or onion greatly increased the amounts of sulfur volatiles from cooked ground beef.

The primary efficacy end point is change in exercise treadmill ti

The primary efficacy end point is change in exercise treadmill time in the treated vs active control patients, with 90% power to detect a 60-second difference in exercise time between cell-treated (n = 200) and active control (n = 100) patients. Key secondary end points include total number of anginal episodes per week and the incidence of independently adjudicated major adverse

cardiac events and serious adverse events. RENEW will be the first adequately powered study aimed at definitively determining the efficacy of a cell therapy (intramyocardially delivered autologous CD34+ cells) for improvement of functional capacity in patients with refractory angina.”
“The purpose of this BKM120 study was to evaluate the behavior of metals, pathogen parasites, and indicator bacteria in sewage effluents during biological HM781-36B ic50 treatment by activated sludge in a wastewater treatment plant in Ribeiro Preto (WTP-RP), Sao Paulo, Brazil. The evaluation was done during a period of 1 year. Results showed that metal concentrations in treated effluents decreased, reaching concentrations according to those established by national regulations. The activated sludge process at the WTP-RP promoted a partial removal of parasites considered as possible indicators according to the WHO

guidelines. Reduction factors varied between 18.2% and 100% for agents such as Endolimax nana, Entamoeba coli, Entamoeba hystolitica, Giardia sp., Ancylostoma sp., Ascaris sp., Fasciola hepatica, and Strongyloides stercoralis. A removal was also observed in total and fecal coliforms quantification. The present study represents an initial evaluation of the chemical and

microbiological removal capacity of the WTP-RP. The results should be of interest for the authorities responsible for the environmental health at municipal, regional, national, and international levels.”
“Purpose of review\n\nIn spite of great scientific advances, cardiovascular disease is the commonest cause of death worldwide and current CCI-779 solubility dmso cardiovascular prevention strategies fail to achieve the full potential of risk modification. A large amount of evidence supports the use of pharmacological treatments both in primary and secondary prevention and it was hypothesized that a fixed-dose combination of such drugs, a ‘polypill’, may greatly simplify and improve current prevention strategies.\n\nRecent findings\n\nSeveral polypill formulations have been developed and a recent trial demonstrated the short-term feasibility, safety and efficacy (in reducing risk factor levels) of a polypill in individuals at moderate risk.

Substitution of the L-proline residue at position 4 of the native

Substitution of the L-proline residue at position 4 of the native peptide with hydroxyproline, valine or D-proline caused a loss of cardioinhibitory activity. Also, replacement of arginine residues at all three positions 2, 7 and 9 with another basic amino acid histidine, reduces cardioinhibitory action of Led-NPF-I. Some modifications www.selleckchem.com/products/BAY-73-4506.html of the C-terminal residues, as the Phe(4-NO2)-, Phe(4-NH2)- and Phe(4-NMe2)-analogues, resulted in agonistic peptides with biological activity similar to that of the native peptide. However,

three other C-terminal analogues tested [Tyr(10)]-, [D-Phe(10)]-Led-NPF-I, and Ala-Arg-Gly-Pro-Gln-Leu-Arg-Leu-Arg-Phe-OH were inactive in the heart bioassay, which suggests that this end of the amino acid chain may play an important role in bioactivity and interaction of the native peptide with its receptor on the myocardium. Copyright (C) 2007 European Peptide Society and BKM120 research buy John Wiley & Sons, Ltd.”
“Robotic lower limb exoskeletons that can alter joint mechanical power output are novel tools for studying the relationship between the mechanics and energetics of human locomotion. We built pneumatically powered ankle exoskeletons controlled by the user’s own soleus electromyography (i.e. proportional myoelectric control) to determine

whether mechanical assistance at the ankle joint could reduce the metabolic cost of level, steady-speed human walking. We hypothesized that subjects would reduce their net metabolic power in proportion to the average positive mechanical power delivered by the bilateral ankle exoskeletons. Nine healthy individuals completed three 30 min sessions walking at 1.25 m s(-1) while wearing the exoskeletons. Over the three sessions, subjects’ net metabolic energy

expenditure during powered walking progressed from +7% to -10% of that during unpowered walking. With practice, subjects significantly reduced soleus muscle activity ( by similar to 28% root mean square EMG, P < 0.0001) and negative exoskeleton mechanical power (-0.09 W kg(-1) at the beginning of session 1 and -0.03 W kg(-1) at the end of session 3; P = 0.005). Ankle joint kinematics returned to similar patterns to those observed during unpowered walking. At the end of the third session, the powered exoskeletons check details delivered similar to 63% of the average ankle joint positive mechanical power and similar to 22% of the total positive mechanical power generated by all of the joints summed ( ankle, knee and hip) during unpowered walking. Decreases in total joint positive mechanical power due to powered ankle assistance (similar to 22%) were not proportional to reductions in net metabolic power (similar to 10%). The ‘apparent efficiency’ of the ankle joint muscle-tendon system during human walking (similar to 0.61) was much greater than reported values of the ‘muscular efficiency’ of positive mechanical work for human muscle (similar to 0.

Here we examined whether chimpanzees show asymmetries in the PT f

Here we examined whether chimpanzees show asymmetries in the PT for grey matter volume and surface area in a sample of 103 chimpanzees from magnetic resonance images. The results indicated that, overall, the chimpanzees showed population-level leftward asymmetries for both surface area and grey matter volumes. Furthermore, chimpanzees that prefer to gesture with their right-handed had significantly greater leftward grey

matter asymmetries compared to ambiguously- and left-handed selleck screening library apes. When compared to previously published data in humans, the direction and magnitude of PT grey matter asymmetries were similar between humans and apes; however, for the surface area measures, the human showed more pronounced leftward asymmetries. These results suggest that leftward asymmetries in the PT were present in the common ancestor of chimpanzees and humans. (C) 2009 Elsevier B.V. Selleckchem Momelotinib All rights reserved.”
“This study aimed to determine the prevalence of psychotic symptoms in urban Tanzania and their relationship with demographic, socio-economic and social factors. A random sample of 899 adults aged 15-59 was surveyed. The main outcome measure was endorsement of one or more psychotic symptoms identified by the Psychosis Screening Questionnaire. 3.9% respondents reported one or more psychotic symptoms in the preceding year. Significantly higher rates of symptoms were found in those who had recently experienced

two or more stressful life events, those with CMD and people who had used cannabis in the preceding year.”
“The aim of this study was to determine if there is a difference between complications for totally implantable central venous catheters (ports) and tunnelled external central venous

catheters (external CVCs) that result in early removal of the central venous catheter (CVC) in children and adolescents with acute lymphoblastic leukaemia (ALL).\n\nAll children hospitalised between November 1996 and December 2007 with ALL who had a CVC were included retrospectively. We analysed data regarding the patient’s first CVC.\n\nWe included 322 patients. 254 received a port and 68 received an external CVC. There were 102 CVC complications that required removal buy PXD101 of the CVC prior to the completion of chemotherapy (65 in patients with ports, 37 in patients with external CVCs). Overall complications requiring CVC removal were significantly less likely to occur in the patient’s with ports (p < 0.001). Ports were significantly less likely to require removal prior to the end of treatment overall (p < 0.001) and for specific complications such as infection (p < 0.001) and dislodgement (p = 0.001). However, when adjusted for disease severity there is no difference in premature CVC removal rates.\n\nWhen patients are risk-stratified for disease severity there is no difference in rates of CVC removal prior to completion of treatment.

Collectively, these experiments highlight the necessity and funct

Collectively, these experiments highlight the necessity and function of multiple related, cytoplasmic host sensors in orchestrating an effective immune response against an acute viral infection.”
“The antiproliferative immunosuppressive drug mycophenolic acid (MPA) is an uncompetitive inhibitor of inosine monophosphate dehydrogenase, a key enzyme in de novo synthesis of purine nucleotides. The latter are not only required for synthesis of DNA and RNA but also are essential for the regulation of numerous cellular signaling pathways modulated by guanine nucleotide binding proteins (G proteins). We undertook an analysis of

the influence of MPA on protein expression in a T-lymphoblast cell line (CCRF-CEM): which displays concentration-dependent inhibition of proliferation by MPA to A-1155463 supplier obtain insight into the influence of MPA on the cellular proteome. Cells were stimulated with phorbol myristate acetate/ionomycin and incubated in the presence

or absence of MPA. Two-dimensional electrophoresis and densitometric imaging revealed 11 differentially expressed protein spots (P < 0.05) on MPA treatment. 6 with increased and 5 with decreased abundance. After find more in-gel tryptic digestion, proteins were identified by quadrupole time-of-flight mass spectrometry. Proteins displaying increased abundance after MPA treatment included splicing factor arginine/serine-rich 2, prostaglandin E synthase 3. peptidyl-prolyl cis-trans isomerase A, and deoxyuridine 5′-triphosphate nucleotidohydrolase. Endoplasmin, proliferating cell nuclear antigen, acidic ALK targets leucine-rich nuclear phosphoprotein 32 family member A, and cofilin I showed decreased abundance after MPA treatment. Three separate spots (I decreased and 2 increased abundance) were identified as Rho guanosine diphosphate dissociation inhibitor 2 (Rho GDI 2) proteins. Western blotting with a monoclonal antibody directed against the Rho GDI 2 site cleaved by caspase 3 demonstrated I spot with increased abundance to be the caspase 3-cleaved product of Rho GDI 2 lacking the first 19 amino acids. Rho GDI 2 plays a central

regulatory role in the activation of Rho guanosine triphosphatases that function as molecular switches in cell signaling pathways affecting cell cytoskeletal dynamics and motility. Our data suggest that MPA can modulate Rho GDI 2 levels in T lymphocytes, thereby potentially disrupting cell signaling pathways important for T-cell function.”
“Antioxidant and radical scavenging properties of a series of 2-[4-(substituted piperazin-/piperidin-1-ylcarbonyl)phenyl]-1H-benzimidazole derivatives were examined. Free radical scavenging properties of compounds 11-30 and 33 were evaluated for the stable free radical 2,2-diphenyl-1 -picrylhydrazyl (DPPH) and superoxide anion radical. In addition the inhibitory effects on the NADPH-dependent lipid peroxidation levels were determined by measuring the formation of 2-thiobarbituric acid reactive substances (TBARS) using rat liver microsomes.

27-0 67) CONCLUSIONS: The MicroRPM reliably measures MIP and MEP

27-0.67). CONCLUSIONS: The MicroRPM reliably measures MIP and MEP, but its MRPD, MRR, and tau measurements should be considered with caution.”
“While bone-patellar tendon-bone (BPTB) autograft continues to be the “gold standard” and most popular graft choice for primary anterior cruciate ligament (ACL) reconstructions, the use of allograft tissues in ACL reconstruction has steadily increased over the last 2 decades. Advantages of allograft include a lack of donor-site morbidity, unlimited available sizes, shorter operative

times, availability of larger grafts, smaller incisions, improved cosmesis, lower click here incidence of postoperative arthrofibrosis, faster immediate postoperative recovery, and less postoperative pain. Disadvantages include the potential for disease transmission and prolonged graft healing. Presented in this article are 2 techniques used at the authors’ institution for primary ACL reconstruction with allograft. With the proper indications, knowledge of graft preparation and handling, and technique, allograft tissues in ACL reconstructions can provide the surgeon with clinical results equal to those of autograft reconstructions.”
“A novel amphiphilic thiolated carboxymethyl chitosan was synthesized. It self-assembled into disulfide bond cross-linked nanoparticles in deionized water. The TEM showed that these nanoparticles had a core-shell

structure with an average diameter of 160nm. Dynamic light find more scattering showed that the nanoparticles were stable in water solution. The particle size changed with pH

PF-03084014 values and GSH concentrations, and reached a maximum diameter at pH 7.0 and 20 mM GSH respectively, exhibiting an obvious pH/redox responsibility. Methotrexate was encapsulated in nanoparticles reaching encapsulation efficiency as much as 43.4%. Release profiles of methotrexate showed a release rate of 19 wt% in pH 7.4 buffer containing 10 mu M GSH, whereas as high as 93 wt% in pH 5.0 buffer containing 20 mM GSH, indicating that the nanoparticles may be used for tumor-specific drug release. The anticancer activity test in vitro showed that the inhibition rate of methotrexate-loaded nanoparticles against HeLa cells reached 90%. (C) 2014 Elsevier Ltd. All rights reserved,”
“The red coloration of litchi fruit depends on the accumulation of anthocyanins. The anthocyanins level in litchi fruit varies widely among cultivars, developmental stages and environmental stimuli. Previous studies on various plant species demonstrate that anthocyanin biosynthesis is controlled at the transcriptional level. Here, we describe a litchi R2R3-MYB transcription factor gene, LcMYB1, which demonstrates a similar sequence as other known anthocyanin regulators. The transcription levels of the LcMYB1 and anthocyanin biosynthetic genes were investigated in samples with different anthocyanin levels. The expression of LcMYB1 was strongly associated with tissue anthocyanin content.

Overall median survival was histology specific (osteosarcoma, 11

Overall median survival was histology specific (osteosarcoma, 11 months; Ewing sarcoma, 26 months; chondrosarcoma, 37 months; chordoma, 50 months) and correlated with extent of local tissue invasion or metastasis at presentation. Presence of metastasis was associated with marked decrease in survival (P < 0.001) for all tumor types. For patients with isolated spine tumors, neoplasms confined within the periosteum were associated with improved overall survival independent of age, radiotherapy, or surgical resection for chordoma (hazard ratio [HR], 0.50; P = 0.08), chondrosarcoma

(HR, 0.62; P = 0.03), Small molecule library and osteosarcoma (HR, 0.68; P = 0.05), but not Ewing sarcoma (HR, 0.62; P = 0.27).\n\nCONCLUSIONS: The preoperative radiographic recognition of local tissue invasion

may identify patients with a more aggressive tumor and help guide the level of aggressiveness in subsequent treatment strategies.”
“A 74-year-old right-handed woman without cognitive impairment suddenly developed nonfluent aphasia. Brain MRI showed acute infarction in the right frontal lobe and insula without involvement of the corpus callosum. A neurological examination demonstrated not only transcortical motor aphasia, but also ideomotor apraxia and right hand predominant constructional apraxia (CA). To date, right hand YH25448 predominant CA has only been reported in patients with corpus callosum lesions. The right hand predominant CA observed in our patient may be associated with the failure to transfer information on the spatial structure from the right hemisphere to the motor cortex of the left hemisphere.”
“gamma-Secretase is an intramembrane-cleaving protease responsible for the generation of amyloid-beta (A beta) peptides. Recently, a series of compounds called.-secretase modulators (GSMs) has been shown to decrease the levels of long toxic A beta species

(i.e., A beta 42), with a concomitant elevation of the production of shorter A beta species. In this study, we show that a phenylimidazole-type JNJ-26481585 GSM allosterically induces conformational changes in the catalytic site of.-secretase to augment the proteolytic activity. Analyses using the photoaffinity labeling technique and systematic mutational studies revealed that the phenylimidazole-type GSM targets a previously unidentified extracellular binding pocket within the N-terminal fragment of presenilin (PS). Collectively, we provide a model for the mechanism of action of the phenylimidazole-type GSM in which binding at the luminal side of PS induces a conformational change in the catalytic center of.-secretase to modulate A beta production.”
“Background Obesity increases the risk of cancer death among postmenopausal women with estrogen receptor-positive (ER+) breast cancer, but the direct evidence for the mechanisms is lacking.

However, low cost development for therapeutic or industrial purpo

However, low cost development for therapeutic or industrial purposes requires, among other properties, that the peptides will be small and with simple buy Ganetespib structure. Therefore, considerable research has been devoted to optimizing peptide length combined with a simple design. This review focuses on the similarities and differences in the mode of action and target cell specificity of two families of small peptides: the naturally occurring temporins from the skin of amphibia and the engineered ultrashort lipopeptides. We will also discuss the finding that acylation of cationic peptides results in molecules with

a more potent spectrum of activity and a higher resistance to proteolytic degradation. Conjugation of fatty acids to linear native peptide sequences is a powerful

strategy to engineer novel successful anti-infective drugs.”
“Distinctive movement of WPD-loop occurs during the catalysis of phosphotyrosine by protein tyrosine Lapatinib datasheet phosphatase 1B (PTP1B). This loop is in the “open” state in apo-form whereas it is catalytically competent in the “closed” state. During the closure of this loop, unique hydrogen bond interactions are formed between different residues of the PTP1B. Present study examines such interactions from the available 118 crystal structures of PTP1B. It gives insights into the five novel hydrogen bonds essentially formed in the “closed”

Danusertib inhibitor loop structures. Additionally, the study provides distance ranges between the atoms involved in the hydrogen bonds. This information can be used as a geometrical criterion in the characterization of conformational state of the WPD-loop especially in the molecular dynamics simulations. (C) 2012 Elsevier B.V. All rights reserved.”
“Aim: To assess the utility of different definitions of the metabolic syndrome (MetS) in predicting adulthood MetS among Tehranian adolescents in a 6-year follow-up.\n\nMethods: A random sample of 2645 adolescents was selected from the Tehran Lipid and Glucose Study (TLGS) in 1999-2001; MetS was assessed based on five definitions for adolescents. In 2005-2007, 572 age 18 years and over were assessed for MetS by NCEP ATP III adults’ criteria. Sensitivity, specificity, and area under receiver operating curve for attaining adulthood MetS, obesity and overweight were calculated for each MetS definitions.\n\nResults: The prevalence of adolescence MetS varied from 0.7 +/- 0.2 to 15.1 +/- 0.8% by different definitions in adolescents and 4.0 +/- 0.9% among adults. The highest area under curve for prediction of adulthood MetS pertained to the de Ferranti’s definition (0.723), and for prediction of adulthood obesity and overweight were 0.723 and 0.606, respectively, for this definition. Kappa for agreement between these definitions was fair (0.195).