Several evidences indicated that single nucleotide polymorphisms (SNPs) in STATs gene such as rs2293152 and rs1053004 at STAT3 and rs7574865 at STAT4 have been associated with chronic hepatitis B (CHB) induced hepatocellular carcinoma (HCC). Objective: This study aims to describe the association between these SNPs and HCC in Thai patients with CHB. Method: Study subjects were enrolled and divided into 3 groups including
CHB-re- lated HCC (n=192), CHB without HCC (n=200) and healthy controls (n=190). The rs2293152 and rs7574865 SNPs were genotyped using polymerase chain reaction – restriction fragment length polymorphism whereas the rs1053004 SNP was genotyped using allelic discrimination assays based on TaqMan real-time PCR. Results: Data analysis revealed that the distribution of rs2293152 and rs1053004 Selleckchem MAPK Inhibitor Library at STAT3 and rs7574865 at STAT4 genotypes were in Hardy-Weinberg equilibrium (P > 0.05). rs2293152 SNP on STAT3 gene was not significantly associated with the risk of HCC
(P > 0.05) whereas the CC genotype of rs1053004 SNP was significantly associated with an increased risk of HCC compared with the CHB without HCC (odds ratio=1.85, 95 %confidence interval=1.00-3.43, P=0.049). In addition, the genotype of rs7574865 SNP at STAT4 (GG versus TT+GT) was significantly associated with a reduced risk of HCC Doxorubicin clinical trial when compared with the healthy controls (odds ratio=1.71, 95 %confidence interval=1.13-2.59, P=0.011). Conclusion: Therefore, these findings provided important
evidence that the rs1053004 SNP at STAT3 and rs7574865 SNP at STAT4 were significantly associated with HCC risk and might be used as a novel genetic marker for HCC in Thai population. Disclosures: The following people have nothing to disclose: Nawin Chanthra, Sunchai Payungporn, Natthaya Chuaypen, Pisit Tangkijvanich Background and Aim: Hepatitis B virus (HBV) has been classified into at 上海皓元医药股份有限公司 least eight genotypes, and the proportion of genotypes varies depending on region. In Japan, HBV genotype C was a most common genotype, while HBV genotype A was rare. But nowadays, the proportion of HBV genotype A is increasing in Japan. Upon infection in adults, HBV genotype A develops chronic infection more often than HBV genotype C. However, the mechanism by which such the difference occur remain unclear. In this study, we investigated the mechanism of the difference of chronicity rates in genotype A and C by using hydrodynamic injection mouse model. Methods: Immu-no-competent NOD mice, NOD-scid mice which are deficient of B and T cells on NOD mice and NOG mice which are further deficient of NK cells on NOD-scid mice, were used. Plasmid pHBA1.2 and pHBC1.2 containing an overlength (1.2-mer) copy of HBV genotype A and genotype C, respectively were transfected by hydrodynamic injection into these mice. Results: Hydrodynamic injection of pHBA1.2 and pHBC1.2 successfully transfected hepatocytes in mice leading to HBV viremia.