These observations are highly coincident with the Ganetespib clinical trial D and I values, which characterize the climate envelope overlap (Table 2). The niche identity tests AZD0156 cell line revealed that the climate envelopes of eastern and western harlequin frogs were identical in terms of annual means of temperature and precipitation. The null hypothesis that climate envelopes are equivalent in the western and eastern ranges was rejected for all other parameters. The climate envelope similarity test revealed that overlap in the ‘annual mean temperature’ and the ‘maximum temperature of the warmest month’ can be most likely traced back to active habitat choice. These findings

corroborate our expectation that climate envelopes of western Baf-A1 clinical trial and eastern Amazonian harlequin frogs show some divergence. However, background effects (i.e. wide availability of suitable climate conditions) may at least partly explain the overlap observed

for the other parameters. Whereas eastern Amazonian Atelopus actively chose their habitats according to some climate components which are only limitedly available to them, these same climate components may be widely available within the range of western Atelopus, where other components may be actually limiting. Such patterns are reasonable since different parameters may be widely available or limiting in eastern or western ranges influencing habitat choice. Hence, our findings suggest once cool-adapted Atelopus ancestors, under warm conditions, were forced to change climate envelopes. Fig. 5 Box plots of seven bioclimatic parameters in climate envelope models of western (W) and eastern Amazonian Atelopus (E) and available climate space within MCPs (W BAC; E BAC). Values given in the upper row refer to temperature in °C and those in the lower row refer to precipitation in mm. Broad horizontal bars indicate the first and third quartiles as well as the Progesterone median. Short horizontal bars indicate minimum/maximum values while dots do represent extremes outside 95% confidence intervals. Mean values

are indicated by crosses Because ‘excellent’ AUC values suggest a high prediction accuracy (see above), we mapped climate envelope of western and eastern Amazonian Atelopus into geographic space on the full presence data point sets (i.e. this time no data points were set aside for testing). Doing so, it is possible to take advantage of all available information and to provide best estimated prediction maps (see Phillips et al. 2006). Results are shown in Fig. 6. Fitting well with the comparison of the climate envelops of the two units studied (Fig. 5; Table 2), their geographic distributions are largely allopatric with overlap corresponding to lower suitability (i.e. lower MaxEnt values). Areas of higher suitability of climate envelopes (i.e. warmer colours in Fig. 6) of western and eastern Amazonian Atelopus show little overlap. Fig.