PrV infection alters multiple biological processes and cellular functions For each time point, the differentially expressed genes from the Qiagen NRSP8 microarray were classified into biological processes using GO terms when available. The biological processes that contained more than 5% of the differentially MEK162 supplier expressed genes during the period between 2 and 8 h pi included protein metabo lism and modification, nucleoside, nucleotide and nucleic acid metabolism, developmental process, signal transduction, trans port, cell cycle, immunity and defense , intracellular protein traffic and cell structure and motility. Several biological processes were predominantly regu lated 1 h pi such as developmental processes and signal transduction.

Other biological processes were regulated later such as cell adhesion or apoptosis from 2 h pi and homeostasis from 4 h pi. The Ingenuity Pathway Analysis of the differentially expressed probes from the Qiagen NRSP8 microarray Inhibitors,Modulators,Libraries identified 82 different top functions associated with sig nificant networks. Three top functions were reg ulated early during infection gene expression, molecular transport and drug metabolism. Inhibitors,Modulators,Libraries Sixteen, 68 and 67 top functions were modulated by PrV infection at 2, 4 and 8 h pi. Fifteen and 14 top functions were specific of time points 4 and 8 h pi, respectively. The number of regulated top functions strongly increased from 4 h pi. The top functions containing the highest number of focus genes at both 4 and 8 h pi were those involved in cancer, cell cycle and cell signaling with the first two detected as early as 2 h pi.

Immune response and immunological dis ease top functions were found from 4 h pi and immune and lymphatic system development and function at 8 h pi. Cell death top function was first detected at 2 h pi. Inhibitors,Modulators,Libraries PrV infection modifies the expression of genes involved in MHC antigenic presentation pathways The expression of many genes belonging to the SLA class I antigenic presentation pathway was modulated during PrV infection according to the results of both microarrays. SLA Ia genes were down regulated from 4 h pi with the SLA PrV microarray and from 8 h pi with the Qia gen NRSP8 microarray. TAP1 and TAP2 genes, encoding molecules involved in peptide transport from the cytosol to the endoplasmic reticulum, were also down regulated 8 h pi according to the results of the Qiagen NRSP8 microarray.

Surprisingly, TAP1 was up regulated 8 h pi with the SLA PrV microarray. PSMB8, one of the genes encoding immunoproteasome Inhibitors,Modulators,Libraries molecules was up regulated Inhibitors,Modulators,Libraries from 4 h pi on the Qiagen NRSP8 selleck chemical Brefeldin A microar ray. Unexpectedly, our results show that transcript levels of genes belonging to the MHC class II antigenic presenta tion pathway were also modulated during PrV infection. Expression of SLA DOB and SLA DMB decreased at 4 h pi according to the results from the SLA PrV microarray.