Together these findings suggest that early immune repression increased hepatocyte permissiveness to infection or enhanced viral replication, resulting in a subsequent elevation in host antiviral learn more immune

and inflammatory activities contributing to fibrogenesis. The overall proinflammatory proteomic profile and concomitant decline in proteins functioning to detoxify potent reactive oxidants suggests that patients with rapidly progressive fibrosis experience greater oxidative stress. We further explored this hypothesis in an independent group of HCV+ liver transplant recipients, demonstrating that patients who develop severe liver disease exhibit a unique metabolic profile characterized by marked changes in compounds associated with alterations in glutathione homeostasis and oxidative stress. The increased expression of gamma-glutamyl peptides is consistent with that described for

a number of liver diseases, including HCV.17, 18, 36 We further observed a decrease in cysteine expression accompanied by an accumulation of amino acids upstream of the cysteine biosynthesis pathway involving CX-4945 CBS, a protein that was less abundant in patients with rapid fibrosis progression. Previous studies demonstrated an important role for CBS in hepatoprotection against oxidative stress and lipid peroxidation and indicated that impaired CBS activity contributes to similar metabolic perturbations during acetaminophen-induced oxidative stress.37, 38 Although we cannot rule out potentially confounding genetic, dietary, environmental, or clinical variables

as an underlying cause, our findings are consistent with previous studies that observed increases in oxidative stress in HCV+ liver transplant recipients relative to nontransplanted patients or control subjects39 and further suggest that HCV+ liver transplant recipients with rapidly progressive fibrosis experience greater levels of oxidative stress 上海皓元医药股份有限公司 relative to their nonprogressor counterparts prior to histologic evidence of liver injury. This provides new insights into a potential role for glutathione homeostasis in HCV pathogenesis, and future studies should examine this connection in greater detail. Alterations in the expression of numerous proteins implicated in fibrogenic processes also occurred prior to histologic evidence of liver disease progression. These include examples of both transcriptionally (LGALS3, IGFBP7) and posttranscriptionally (FAM3C) regulated protein abundance increases.