To further test the idea that exposure to natural sounds influences the maturation of songbird auditory circuits, songbirds can be reared and tutored with either conspecific or heterospecific song. The intent of this manipulation is to alter the acoustics rather than the amount of auditory experience. One

study has shown that the responses of single central auditory neurons display higher information coding capacities and firing rates in male birds tutored Tanespimycin clinical trial with conspecific song, as compared to birds tutored with heterospecific song, demonstrating that experience and vocal learning are linked with auditory development (Figure 8; Woolley et al., 2010). The influence of this kind of experience on development of perceptual skills such as discriminating among the unique songs of individual birds is still unknown. The maturation of

learning, itself, provides another unique opportunity to assess auditory coding properties. As discussed above, auditory training commonly improves adult performance, but perceptual learning is poor in young animals (Sarro and Sanes, 2010 and Huyck and Wright, 2011). Although the effects of learning have yet to be assessed in the developing auditory CNS, a study in finches has shown that, at the level of sound production, forebrain motor neurons display increased temporal precision and rate as finches practice their songs (Crandall et al., 2007). Even before hair cells are activated by sound, they discharge spontaneously and release transmitter, thereby eliciting Selleckchem Perifosine bursts of action potentials in primary auditory neurons, which then excites central circuits (Tritsch et al., 2010 and Johnson et al., 2011). The presence of spontaneous activity prior to sensory activation has been implicated in the maturation of synaptic connections in the visual system (Feller, 2009) and may serve a similar role in auditory development. For example, inhibitory projections Sclareol from the medial nucleus of the trapezoid body (MNTB) to the lateral superior olive (LSO) undergo a dramatic period of functional refinement before the onset of hearing (Kim and Kandler, 2003). During this

period, MNTB afferents release three transmitters (GABA, glycine, glutamate), and disruption of glutamate release prevents functional refinement (Gillespie et al., 2005 and Noh et al., 2010). A current model suggests that the prehearing period of spontaneous activity-dependent synapse maturation may lead to a second phase during which silenced synapses are anatomically eliminated (Kandler et al., 2009). Thus, activity-dependent plasticity occurs well in advance of acoustic experience. Our emphasis on the prolonged time course of perceptual maturation implies that certain synaptic or biophysical properties must also be late developing. However, studies that chart the maturation of these cellular properties generally report that maturation occurs rapidly, usually within 7–14 days of hearing onset, in rodents.