The strains and their corresponding Genbank accession numbers are shown … Table 1 Classification Tofacitinib alopecia and general features of A. jilinensis Y1T according to the MIGS recommendations  Figure 2a Transmission electron micrograph of cells of strain Y1T, showing a longitudinal ultrathin section of a cell forming a spore. Bar: 0.2 ��m (a). Figure 2b Transmission electron micrograph of cells of strain Y1T, showing a longitudinal ultrathin section of the peritrichous flagella in the stationary phase of growth. Bar: 0.5 ��m (b). Genome sequencing information Genome project history The genome of A. jilinensis was selected for next-generation sequencing on the consideration of its facultatively anaerobic characterization and as a new member in genus Amphibacillus.
This is the first genome report for any of the eight Amphibacillus species. Two others are the subject of ongoing own genome projects. This Whole Genome Shotgun project of A. jilinensis was deposited at DDBJ/EMBL/GenBank under the accession “type”:”entrez-nucleotide”,”attrs”:”text”:”AMWI00000000″,”term_id”:”409188581″,”term_text”:”AMWI00000000″AMWI00000000 and consists of 83 contigs (further assembling constructed these contigs into 30 scaffolds). Table 2 presents the project information and its association with MIGS version 2.0 compliance . Table 2 Project information Growth conditions and DNA isolation A. jilinensis Y1T was cultivated aerobically in modified JY medium, which contains (per liter distilled water) 2.0 g yeast extract (Difco), 5.0 g sucrose, 0.2 g KCl, 0.2 g KH2PO4, 0.1 g MgCl2. 6H2O, 0.
5 g NH4Cl, 0.1 g CaCl2, 0.06 M NaHCO3 and 0.44 M NaCl, final pH 9.0 at 32��C for 3 days . Genomic DNA was extracted using the method described by Marmur . The yield, purity and the concentration of genomic DNA was judged by the 0.7% agarose gel electrophoresis with ��-Hind III digest DNA Marker (TaKaRa, Dalian, China) and measured by the NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific Inc., USA). About 736.6 ��g genomic DNA at the concentration 744 ng/��l was obtained. Genome sequencing and assembly Genomic DNA sequencing of A. jilinensis Y1T was performed using Solexa paired-end sequencing technology (HiSeq2000 system, Illumina, Inc., USA)  with a whole-genome shotgun (WGS) strategy, with a 500 Cilengitide bp-span paired-end library (~500 Mb available reads, ~130-fold genome coverage) and a 2,000 bp-span paired-end library (~250 Mb available reads, ~65-fold genome coverage). All these clean reads were assembled into 83 contigs (the minimum length is 231 bp) and 30 scaffolds (the minimum length is 542 bp) using the SOAPdenovo v.1.05 [30,31,50]. The quality of the sequencing reads data was estimated by G+C content and sequencing depth correlation analysis.