Methods: In experimental group, cells were treated with lipopolysaccharide (LPS) for 2 h. No LPS was treated in the control group.
Total RNA was extractedand used for miRNA microarray analysis. The predicted targets of miRNA with significant change were obtained using miRBase. To assess the function of predicted target gene lists, we evaluated the frequency of specific gene ontology (GO) terms among the predicted target genes of the miRNA with significant change using DAVID (Database for Annotation, Visualization and Integrated Discovery).
Results: After normalization, the number of the differentially expressed genes was 15. Among them, 5 miRNAs were up-regulated and 10 miRNAs were down-regulated in LPS group compared with control RG7112 group. The most enriched GO terms in the predicted target genes of miRNA with increased expression were developmental process, response to biotic stimulus, acute inflammatory response, and regulation of cell growth. The most enriched GO terms in the predicted target genes of miRNA with decreased expression were developmental process, cell differentiation,
endocytosis, cell communication. I kappa B kinase/NF kappa B cascade, complement activation, innate immune response and cell adhesion.
Conclusions: In conclusion, we identified the differentially expressed miRNA in LPS induced acute inflammation of HMEECs whose expression profile may provide a useful
ASP2215 Protein Tyrosine Kinase inhibitor clue for the understanding of pathophysiology GANT61 molecular weight of otitis media. Our work indicates that miRNA play important role in the pathogenesis of otitis media. (C) 2011 Elsevier Ireland Ltd. All rights reserved.”
“Background and Purpose: Complete stone removal is important in upper tract stone surgery. Unfortunately, even with the latest technologic advances, current methods only achieve 50% to 80% complete clearance of upper tract stones at the time of primary treatment. Our group has explored the novel use of peptide-coated iron oxide superparamagnetic microparticles that bind to calcium stones, allowing for extraction of these stones with magnetic tools. We present analytic and numeric models that characterize stone attraction performance for feasible magnetic tool sizes and stone magnetization levels.
Materials and Methods: Magnetostatics equations are applied to a simplified, one-dimensional scenario of a spherical target coated with a variable amount of superparamagnetic particles, placed under the influence of a magnetic field aimed at vertical attraction (capture) of the target. Equations are parameterized in terms of (a) target size, ranging from 0.5 mm to 3 mm to represent stone sizes of interest, (b) effective magnetization per surface area delivered by the particle binding chemistry, and (c) distance to the field source.