Knowing the VWF:RCo activity is essential for identifying, subtyping and monitoring VWD, but the assay is poorly standardized and many protocols do not fulfil the clinical need in all situations. This has led to the development of novel activity assays, independent of ristocetin, with enhanced assay characteristics. Results from the

first independent clinical evaluations are promising, showing that they are reliable and suitable for VWD diagnosis. The qualitative type 2 VWF deficiency can be further divided into four different subtypes (A, B, M and N) using specific assays that explore other activities or the size distribution of VWF multimers. These methods are discussed check details herein. However, in a number of patients it may be difficult to correctly classify the VWD phenotype and genetic analysis may provide the best option to clarify the disorder, through mutation identification. von Willebrand factor (VWF) is a multimeric glycoprotein, synthesized by endothelial cells and megakaryocytes. It is important for platelet adhesion to the subendothelium and for platelet–platelet

interactions, and it is the specific carrier of factor VIII (FVIII) in plasma. von Willebrand disease (VWD) is heterogeneous because molecular see more defects can occur in more than one of the functional domains of the multimeric glycoprotein [1, 2]. These functions are explored by an array of laboratory assays, but no one assay reflects the whole spectrum of VWF activities. VWD is classified into three different types: partial or complete VWF quantitative deficiencies (types

1 and 3) and qualitative this website deficiency (type 2). Tests for the correct diagnosis of VWD must assess the most important VWF properties: antigenic level of VWF (VWF:Ag); VWF-platelet GPIb interaction (VWF:RCo); VWF-subendothelium-collagen interaction (VWF:CB); VWF-FVIII interaction (VWF:FVIIIB); and the capacity of VWF to be organized into multimers. FVIII activity (FVIII:C) is also included in the diagnostic work-up because it reflects the ability of VWF to protect FVIII from degradation and is a useful complement in patients with suspected type 2N variants. Prior to laboratory tests, the diagnosis and appropriate classification of VWD requires evidence of a bleeding history, usually also present in other family members. The physician should take into consideration the practical advantage and the patient perspective of a specific diagnosis of VWD in any given patient, avoiding the risk of over-medicalization of patients with dubious or mild bleeding histories [3]. Written bleeding questionnaires are increasingly used to improve the quality of data collection and to reduce both intra- and inter-observer variability.