K. High magnification view of the IR and IL. L. High magnification view of the VR in E. (G-L, bars = 200 nm). Figure 8 Transmission electron micrographs (TEM) of Calkinsia aureus showing the feeding apparatus. The ventral flagellum was disorganized in all sections (A-D at same scale, bar = 1 μm; E-G at same scale, bar = 1 μm). A. Section showing the oblique striated fibrous structure (OSF) and the VR along the wall of the flagellar pocket (FLP). Arrow points out the LMt and the DL. B. Section through the congregated globular structure (CGS), the OSF Epacadostat supplier and the feeding pocket (FdP). The VR extends to the right. The arrow points out the LMt and the DL,

which extend from the VR to the IR and support the dorsal half of the FLP. C. Section showing the VR over the CGS. Arrows show the LMt and DL. D. The VR crosses over the CGS and extends to right side of the FdP. Most of the wall of the FLP is supported by the LMt and DL (arrows). E. A striated fiber (double arrowhead) supports the left side of the FdP and extends from the left side of the CGS. Arrows indicate the extension of the LMt and DL. F. Section through the beginning of the vestibulum (V) and the striated

fiber (double arrowhead). G. The V is enlarged and the CGS remains at both sides of the FdP. H. High magnification of FdP. I. Tangential TEM section showing ACP-196 mw the VR with an electron dense fiber along the feeding pocket and a tomentum (T) of fine hairs. J. Longitudinal section through the CGS

and the OSF. Six ventral root microtubules embedded within the electron dense fibers (arrowheads). K. High magnification view of the VR supporting the FdP shown in F. Double arrowhead indicates the striated fiber and the six arrowheads indicate the electron dense fibers of the VR. (H-K, bars = 500 nm). Figure 9 Diagram of the cell (A), the flagellar apparatus (B) and the feeding apparatus (C) of Calkinsia aureus based on serial TEM sections. A. Illustration of the cell viewed from the left side; arrow marks the extrusomal pocket. Boxes B and C indicate the plane also of view shown in 4EGI-1 concentration Figures B and C, respectively. B. Illustration of the flagellar apparatus as viewed from left side. C. Illustration of the feeding apparatus as viewed from anterior-ventral side. The double arrowhead marks the striated fiber along the feeding pocket (FdP). Note DL, IF, IL, LF, LMt, and RF are not shown on this diagram for clarity. Flagella, Transition zones and Basal Bodies Both flagella contained a paraxonemal rod adjacent to the axoneme, and flagellar hairs were not observed on either flagellum (Figure 6A). The paraxonemal rod in the dorsal flagellum (DF) had a whorled morphology in transverse section, and the paraxonemal rod in the ventral flagellum (VF) was constructed of a three-dimensional lattice of parallel fibers (Figures 6B, 6K). The entire length of the axoneme had the standard 9+2 architecture of microtubules (Figure 6B).