In this contribution, the potential of nondestructive Raman spectroscopy for the detection, quantification and discrimination of heme and heme degradation products is investigated. Resonance Raman spectroscopy using different excitation wavelengths (413, 476, 532, and 752 nm) is employed to estimate

the limit of detection for hemin, myoglobin, biliverdin, and bilirubin. Concentrations in the low micromolar range (down to 3 mu mol/L) could be reliably detected when utilizing the resonance enhancement effect. Furthermore, a systematic study on the surface-enhanced Raman spectroscopy (SERS) detection of hemin in the presence of other cellular components, such as the highly similar cytochrome Liproxstatin-1 c, DNA, and the important antioxidant glutathione, is presented. A microfluidic device was used to reproducibly create a segmented flow of aqueous droplets and oil compartments. Those aqueous droplets acted as model chambers where the analytes have to compete for the colloid. With the help of statistical analysis, it was possible to detect and differentiate the pure substances as well as the binary mixtures and gain insights into their interaction.”
“Objective: In a study to identify an early hemodynamic predictor of syncope, 12 men (25-40 yr) underwent 30 min HMR-1275 of 80 degrees head-up tilt, followed by progressive lower

body negative pressure (LBNP) until presyncope. Methods: Temporal (supplying extracranial tissues: TEMP), middle cerebral (MCA), and superficial femoral (FEM) arterial flow velocity (V) and vascular resistance indices (VR) were evaluated continuously using Doppler ultrasound. Ratios of the Doppler V-MEAN (V(MCA)N(FEM)or V-MCA/V-TEMP) were used to assess flow redistribution between these areas. Results: The progression of the testing protocol showed increases in vascular resistance in all territories. At presyncope, both MCA(VR) and FEMVR were reduced while there was a large increase in TEMPVR Vasoconstriction of the vascular bed supplied by the temporal artery occurred early during central hypovolemia resulting in the appearance

of negative velocity deflections, check details which could be used for the early detection of impending syncope. Analysis of the velocity ratios showed little change until the onset of presyncope where there was an increase in VMCANTEMP which confirmed that vasoconstriction of the vascular bed supplied by the TEMP artery contributed to cardiac output redistribution in favor of the brain, and a reduction in VMCANFEM suggesting a redistribution of cardiac output toward the legs. Discussion: In 67% of the tests, the appearance of the negative component of V-TEM was an early sign of increasing TEMPVR that occurred before visually detectable changes in V-FEM or V-MCA and within 5 min before presyncope. Such easily identifiable in real time Doppler signs allowed experimenters to anticipate test termination.