morphological resemblance has caused much confusion and isolates are often misidentified or not differentiated by taxonomists using morphological and physiological techniques (Pitt et al. 1990). Sixty-nine strains originating from cork and belonging to the Glabra series were grouped according to their partial β-tubulin gene sequences. A subset of these strains was selected for macro- and microscopic analysis, extrolite profiling and sequencing a part of the β-tubulin and calmodulin gene. In addition, PI3K Inhibitor Library research buy ex-type strains of various related species were included in the analysis. Our polyphasic taxonomic approach shows that a group of isolates share peculiar differences with other known species, and a new species is proposed for this group of isolates. Materials and methods Fungal strains For our taxonomic study, a selection of these sixty-nine
strains isolated from cork, was made and supplemented with related (ex-type) strains (Table 1). Spore suspensions of the cultures were maintained in 20% glycerol at −80°C. Table 1 List of isolates belonging to Series Glabra and related Penicillia CBS no. Other no. Name Remarks CBS 235.60 ATCC 18483 = FRR 634 E. pinetorum Ex-type selleck inhibitor of P. silvaticum; forest soil, USSR CBS 295.62 ATCC 14770 = CCRC 31517 = DSM 2438 = IFO 7743 = IMI 094209 = MUCL 31196 = NRRL 3008 E. pinetorum Ex-type; soil, conifer and hardwood forest, Wisconsin, USA CBS 260.29 IMI 092242 = NRRL 774 = Thom4733.60 P. glabrum Ex-type of P. flavidorsum; unrecorded source CBS 213.28 FRR 770 = IMI 092265 = IMI 092265ii = NRRL 770 P. glabrum Ex-type of P. oledzskii; soil under conifer, Poland CBS 344.59 http://www.selleck.co.jp/products/Gemcitabine(Gemzar).html ATCC 18486 = IFO 5359 = IMI 068617 = NRRL 3460 P. glabrum Ex-type P. spinuloramigenum; butter, Japan CBS 228.28 FRR 752 = IMI 092232 = MUCL 29114 = NRRL 752 P. glabrum Ex-type of P. terlikowskii; soil under conifer, Poland CBS 229.28 FRR 751 = IMI
092231 = MUCL 29111 = NRRL 751 P. glabrum Ex type of P. paczowskii; soil under conifer, Poland CBS 105.11 P. glabrum Ex-type of P. frequentans; unknown substrate, Germany CBS 127700 P. glabrum Non-boiled cork CBS 127701 P. glabrum Cork, after the 1st boiling process CBS 126333 P. glabrum Cork discs CBS 127702 P. glabrum Non-boiled cork CBS 127703 P. glabrum Non-boiled cork CBS 127704 P. glabrum Non-boiled cork CBS 127705 P. glabrum Non-boiled cork CBS 126336 P. glabrum Non-boiled cork CBS 125543 IBT 22658 P. glabrum Ex-type; unrecorded source CBS 687.77 IJFM 3745 = IMI 253783 P. grancanariae Ex-type of P. grancanariae; air, Gran Canaria, Spain CBS 336.79 ATCC 38669 = IJFM 3840 = VKM F-2181 P. palmense Ex-type; air, Gran Canaria, Spain CBS 126.64 P. purpurescens Soil, Erzurum, Turkey CBS 366.48 ATCC 10485 = IMI 039745 = NRRL 720 = QM 1959 P. purpurescens Neotype; soil, Canada CBS 328.48 ATCC 10444 = IMI 040234 = NRRL 1915 P. spinulosum Ex-type of P.