For example, the dynamic TNO-gastrointestinal system (TIM-1) of the human small intestine combined with the Caco-2 cell model was used to investigate the digestive stability and intestinal

absorption of lycopene and α-tocopherol [7] Furthermore, adhesion to and cytokine expression of Caco-2 cells was assessed using bacterial cultures, including the probiotic strain Bifidobacterium longum DD2004, obtained from a three-stage continuous-culture system (CCS) simulating the proximal and distal large intestine [8]. Results clearly indicate that application of fermentation effluents to intestinal cells represents a valuable platform for assessing epithelial responses as a function of in vitro fermentative processes learn more and Selleckchem FHPI microbial interactions. In this Mocetinostat purchase study, a three-stage continuous intestinal fermentation model closely mimicking conditions in the proximal, transverse and distal colon

regions and inoculated with immobilized child feces was used to generate a complex microbiota. For the first time, we report the effects of Salmonella in a complex gut microbiota containing metabolites and grown under environmental conditions of the different sections of the colon, on mucus-secreting intestinal HT29-MTX cells. This combined model approach was used to assess host-protecting, anti-Salmonella activities of probiotic and prebiotic combinations. Mean invasion efficiencies of S. Typhimurium N-15 into HT29-MTX cells measured in colonic effluents were up to 50-fold lower compared to values measured in simple experimental conditions of a single Salmonella strain in DMEM, reflecting different microbe cell interactions in simple systems compared to environments with a complex gut microbiota [24]. Bacterial interactions occurring at

the brush-border of HT29-MTX cells may enhance barrier function and diminish Salmonella invasion capacity, through the presence of a complex host microbiota, specific metabolites, as well as competition for adhesion sites. SCFAs at physiological concentrations are known to induce a concentration-dependent, reversible change in cellular permeability in vitro [25, 30]. A higher concentration of total SCFAs in fecal water of adults applied to Caco-2 cells was shown to be associated with an increase in TER in comparison to fecal water obtained from Farnesyltransferase elderly subjects containing lower SCFA concentrations which negatively affected epithelial barrier function [31]. Our results obtained with effluents sampled at the end of model stabilization periods (Stab) were in accordance with these findings. Indeed, a generally higher TER across HT29-MTX cell monolayers was measured after 24 h of incubation for transverse and distal reactor samples with a high concentration of SCFAs accumulating in the in vitro model due to the lack of absorption, compared to samples from the proximal reactor.