For detailed cluster contents and OTU annotations, see Additional file 2 Table S1. Figure 4 Pair-wise comparison of fungal species richness in water-damaged and reference buildings pre- to post-remediation. Phylotype diversities (Sn) were calculated from clone library data separately for each sample and for each fungal class. The diversity ratio between the index and reference buildings (Sn(In):Sn(Re)) was calculated for each building pair pre- and post-remediation. The LCZ696 concentration results for buy GDC-0941 the two locations are shown separately. The species

richness of Agaricomycetes, Eurotiomycetes and Dothideomycetes was higher in the index buildings in relation to reference buildings’ pre-remediation, but decreased post-remediation. Table 1 shows the ERMI values derived from the qPCR data. These were higher for the index buildings (4.0 and 4.4) and lower for the reference buildings (-5.2 and -1.3). The following group 1 ERMI assays were responsible for elevated values in the index buildings: Wsebi, PvarB, Tviri (Index-1) and PenGrp2 (Index-2). Occurrence of material-associated fungi in dust A total of 45 fungal selleckchem phylotypes

were detected from the building material samples collected from the two index buildings. An in silico analysis showed that 13 of the phylotypes (29%) had a matching sequence with the qPCR targets (see Additional file 7 Table S6 for targeted species). Eight of the 45 phylotypes were detected in the dust samples MG-132 mw in corresponding buildings using clone library analysis or qPCR. These were C. cladosporioides, C. herbarum, Eurotium sp., P. chrysogenum, P. herbarum, P. chartarum, T. atroviride and W. sebi. Most of

these were ubiquitous in both the index and reference buildings’ dust samples. The summed qPCR cell counts for these fungi were similar in the index and reference building pairs; together, the species accounted for 3.8 × 105/8.0 × 105CE g-1 and 6.4 × 105/6.7 × 105CE g-1 in the index/reference buildings in Location-1 and Location-2, correspondingly. Three individual taxa, L. chartarum, T. atroviride and W. sebi occurred exclusively, or in substantially higher numbers, in an index building than the corresponding reference building (Additional file 2 Table S1). Penicillium chrysogenum was abundant only in the index building according to clone library analysis, but qPCR reported equally high numbers of this species in both the reference and the index buildings.