Etymology: ‘aethiopicum’ refers to the country where this species was first discovered, Ethiopia. Habitat: Soil Known distribution: Ethiopia. Holotype: Ethiopia, Welega Prov., isolated from soil under coffee, date unknown, T. Mulaw (BPI 882291; ex-type culture C.P.K. 1837 = G.J.S. 10–166 = CBS 130628). tef1 = EU401615, cal1 = EU401483, chi18-5 = EU401534, rbp2 = HM182986. Additional cultures examined: Ethiopia,
Harerga, isolated from soil under coffee, date unknown, T. Mulaw (C.P.K. 1841 = G.J.S. 10–167. Sequences: tef1 = EU401616, cal1 = EU401484, chi18-5 = EU401535); Jimma, isolated from soil under coffee, date unknown, T. Mulaw (CBS 130627 = C.P.K. 1817 = G.J.S. 10–165. Sequences: tef1 CP-690550 cell line = EU401614, cal1 = EU401482, chi18-5 = EU401533). Comments: Trichoderma aethiopicum is a member of a clade that includes T. longibrachiatum, RG7112 cell line H. orientalis, the new species T. pinnatum, and the strain CBS 243.63. The two common species in this clade, T. longibrachiatum and H. orientalis, are pantropical, whereas the other species in
the clade AZD1390 price appear to be Paleotropical/Australasian endemics. Trichoderma aethiopicum is known only from three strains isolated from soil under coffee in Ethiopia. There is no practical way to distinguish most of these species on the basis of their physical phenotype, although conidia of T. aethiopicum have a somewhat larger length/width ratio than T. longibrachiatum or H. orientalis. Strain CBS 243.63 (Fig. 5) has larger conidia than any of the members of this clade [(3.7–)4.7–7.7(−10.2) × (2.0–)2.7–3.5(−3.7) Pregnenolone μm]. This strain was derived from ascospores of a Hypocrea collection made early in the 1960’s in New Zealand by J.M. Dingley and sent to J. Webster in the UK; that collection cannot be located. The culture appears to be degenerated. While this strain clearly represents a distinct lineage within the Longibrachiatum/Orientalis subclade, we are not confident that we can adequately characterize it. We deposit sequences in GenBank in the hope that the species will be recognized in the future. Fig. 5 Trichoderma sp. CBS 243.63. a Pustules from CMD. b–e, f Conidiophores
and phialides. f, g Conidia. Intercalary phialides indicated by arrows. h. Chlamydospores. i. Colony 1 week on PDA under light just beginning to sporulate. b, f from CMD; b–e, g, h from SNA. Scale bars: a = 2 mm, b–e, h = 20 μm. g = 10 μm 2. Hypocrea andinensis Samuels & O. Petrini in Samuels et al., Stud. Mycol. 41: 13 (1998). Anamorph: Trichoderma sp. Ex-type strain: G.J.S. 90–140 = CBS 354.97 = ATCC 208857 Typical sequences: ITS X93957, tef1 AY956321 This species was described (Samuels et al. 1998) based on a single perithecial collection made in the Venezuelan Andes at an elevation of 2,300 m. Since then we have examined soil cultures from Saudi Arabia (G.J.S. 01–355), Amazonian Peru (G.J.S. 09–62, San Martín State) and Hawaii (C.P.K.