Each group contained 10 animals. Before administration of drug, apparent health of these animals was monitored during the conditioning period under the laboratory environments for a week before administration of algal extract specifically noticing loss of hair, diarrhea, edema, ulceration and lack of activity. Diet Tyrosine Kinase Inhibitor Library nmr and water was provided ad libitum. The animals were maintained under constant environmental conditions 23 ± 2 °C. All animals were given standard diet prepared in the laboratory and water ad libitum for 30 days. They were housed individually in transparent cages, in a quiet room,

under controlled condition of temperature atleast a week before the beginning of experiments, for acclimatization with the environment. The

dosing of Iyengaria stellata was done daily. Ethanolic extract PS-341 clinical trial of seaweed was suspended in distilled water dist.H2O and administered orally at 10 mg/200 g body weight daily for 30 days to the animals of the test group, while the same quantity of dist.H2O was given orally to the animals of the control group. The doses were adjusted according to the body weight of individual animal. 20 All animals received drugs orally. Body weight was monitored weekly. Animals were handled as per specifications provided in Helsinki Resolution 1964 and study was approved by our Board of Advanced studies and research vide Resol. No, 1135 dated: 20-04-2011-22 & 27-04-2011. Blood 2 ml will be collected in EDTA.K3 tubes for blood

hematological examination e.g. erythrocyte count RBC, white blood cell count WBC, Platelet count PLT, hemoglobin Hb on automatic Humacount plus 3 part differential with histogram. Hematology analyzer. Model # 16400/S. Human Germany.25 All values are compared with the controlled and standard drug by taking mean of all of them and the significance of difference between means is determined by student significance t-test. Cediranib (AZD2171) Values of P < 0.05 is considered as significant. Effect of seaweed on blood parameters is shown in Table 1. Various research studies on the therapeutic effects of alga have been conducted in order to prevent and cure different ailments. In the current study brown algae Iyengaria stellata has been explored for its hematopoietic effect. Literature survey revealed that polysaccharide is the major component responsible for the hematopoietic effect. The hematopoietic activity is through the stimulation of secretion of interleukin-6 and GM colony-stimulating factor, and the amounts of these hematopoietic growth factors secreted, in general, agreed with the number of GM colony formations. 26 Other studies using >99% pure carbohydrate fraction from Aloe vera extracts revealed increased hematopoietic and hematologic activity compared to the starting material.