Conclusion: In summary, our results of this study demonstrate that BDNF is able to enhance contractile activity of the isolated intestinal tracts of mice acutely and directly. The mechanism of this effects is that BDNF binds to TrkB dimers to activate the PLC pathway, which results in the formation of the second messengers DAG and IP3 and calcium release from intracellular stores. The

evaluation of intracellular calcium may induce BDNF’s effect on gut motility. However, the details of the mechanism shoule be investigated. Additionally, present study combined with previous studies suggest that BDNF might be worth reevaluating to explore the therapeutic potential in patients with disturbed gut motility, such as Hirschsprung’s disease, slow-transit constipation, or C-IBS. Key Word(s): 1. BDNF; 2. intestinal strips; 3. TrkB; Selleck AZD1208 4. mice; Presenting Author: ZHANG YONG-GUO Additional Authors: GUO XIAO-ZHONG, LI HONG-YU, LI XUE-YAN, SHAO XIAO-DONG Corresponding Author:

GUO XIAO-ZHONG Affiliations: General Hospital of Shenyang Military Area Command; General Hospital of Shenyang Military Area Command Objective: To investigate the effect of miR-187 on the cell proliferation, cell apoptosis and metastasis of colon cancer LOVO cell. Methods: MiR-187 mimics was transfected into LOVO cell. MTT assays, flow cytometry assay, cell invasion and migration assays were performed to evaluate the AZD1152-HQPA nmr effect of miR-187 on the cell proliferation, cell apoptosis and metastasis of colon cancer LOVO cell. Results: As indicated by MTT assay, compared with control cells,

GNA12 miR-187 mimics transfected LOVO cells showed a significantly increased rate of cell proliferation. Flow cytometry assay showed a decreased apoptotic index of miR-187 mimics transfected LOVO cells. Cell invasion and migration assays suggested miR-187 could promote the migration and invasion of LOVO cell in vitro. Conclusion: MiR-187 promotes the proliferation and metastasis of LOVO cells. These results suggest miR-187 play an important role in the malignant phenotypes of colon cancer. Key Word(s): 1. miR-187; 2. colon cancer; 3. proliferation; 4. apoptosis; Presenting Author: ZHANG YONG-GUO Additional Authors: GUO XIAO-ZHONG, LI HONG-YU, LIU XU, YAO HUI Corresponding Author: GUO XIAO-ZHONG Affiliations: General Hospital of Shenyang Military Area Command Objective: Deleted in breast cancer 1 (DBC1) was initially cloned from a region homozygously deleted in breast cancers, but its role in colorectal cancer remains unknown. The present study aims to examine the expression level of DBC1 and assess its prognostic value in human colorectal cancer. Methods: Immunohistochemical staining was performed to detect the expression level of DBC1 in a series of 186 colorectal cancer patients. Immunohistochemical staining results were analyzed and compared statistically with various clinicopathological characters and overall survival.