But, in most of the cases, it failed due to linkage drag of undesirable plant and pod features. Identification of tightly linked molecular markers will help to identify the desirable

recombinants more efficiently. A recombinant inbred line population comprising 164 lines was developed from a cross between a rust-resistant parent VG 9514 and a rust susceptible parent TAG 24. Using a modified bulk segregant analysis, 243 transposable element (TE) primer pairs were screened for putative linkage with rust resistance. Of the 243, 40 TE primer pairs were found polymorphic between parents and two transposable element markers, and TE 360 and TE 498 were found associated with rust resistance gene. Based on genetic mapping, TE 360 was found linked to the rust resistance gene at 4.5 cM distance. Identification Selleck Everolimus of such markers could be applied for marker-assisted selection of rust resistance plants in peanut. “
“Eggplant (Solanum melongena L.) plants with severe leaf mosaic and mottling were found in a kitchen garden near cotton fields in Pakistan. Rolling Circle Amplification products from six of the naturally infected eggplant plants, subjected to PCR, successfully amplified expected products of 2.8 and 1.4 kb using begomovirus and betasatellite-specific primers, respectively. Based on 99% nucleotide sequence identity, the virus was identified as a variant of Cotton leaf curl Burewala virus (CLCuBuV) (GenBank Accession No. HG428709). Likewise,

the sequenced betasatellite with a maximum INCB018424 solubility dmso of 97% nucleotide sequence identity was recognized as a new variant of Cotton leaf curl Multan betasatellite (CLCuMuBMul) (GenBank Accession No. HG428708). selleck products The symptomatic induction of Cotton leaf curl disease in CLCuBuV susceptible cotton genotype CIM-496 by back-indexing further confirmed the presence of CLCuBuV in eggplant. This is the first report of CLCuBuV and its associate betasatellite in naturally infected plants of eggplant. “
“Rice stripe virus (RSV), a member of the genus Tenuivirus, causes

rice stripe disease in East Asia and is one of the most economically important rice pathogens. The pathogenesis of RSV and the molecular basis of plant responses to the pathogen are poorly understood. We investigated the process of RSV infection in Arabidopsis thaliana which is highly susceptible to the virus. A simple inoculation method using viruliferous small brown planthoppers was developed to infect A. thaliana plants with RSV. The symptoms were developed within 2 weeks of inoculation. One month after inoculation, all infected plants showed stunted growth and vein chlorosis in newly emerged leaves. Forty-five days after inoculation, RSV-infected plants showed severely stunted growth and distorted flower stalks. RSV replication in A. thaliana was confirmed using a dot immunobinding assay, reverse transcription-polymerase chain reaction, and a protein gel blot assay. RSV infection strongly induced PR1, PR2 and GST1 but not PDF1.