At the 6-h and 24-h time points, there was evidence of a minor amount of radioactive material that appeared to be 6-fluoro-6-deoxy-D-sorbitol and possibly 6-fluoro-6-deoxy-D-gluconic acid.
Conclusion: On the time scale typical of PET imaging studies radioactive metabolites of [(18)F]6FDG
are negligible. (C) 2011 Elsevier Inc. All rights reserved.”
“Introduction: The present investigation focuses on the chemical and biological fate of Zr-89 in mice. Electrophoreses of Zr-89 solvated or chelated in different conditions are here presented. The biological fate of mice injected with [Zr-89]Zr-oxalate, [Zr-89]Zr-chloride, [Zr-89]Zr-phosphate, [Zr-89]Zr-desferrioxamine and [Zr-89]Zr-citrate is studied with the biodistribution, the clearances and positron emission tomography images. A special focus Selisistat clinical trial is also given regarding the quality of Zr-89 bone accumulation.
Methods: Electrophoreses were carried GKT137831 price out on chromatography paper and read by gamma counting. Then, the solutions
were intravenously injected in mice, imaged at different time points and sacrificed. The bones, the epiphysis and the marrow substance were separated and evaluated with gamma counts.
Results: The clearances of [Zr-89]Zr-chloride and [Zr-89]Zr-oxalate reached 20% of injected dose (ID) after 6 days whereas [Zr-89]Zr-phosphate was only 5% of ID. [Zr-89-]Zr-citrate and [Zr-89]Zr-DFO were noticeably excreted after the first day postinjection (p.i.). [Zr-89]Zr-chloride and [Zr-89]Zr-oxalate resulted in a respective bone uptake of similar to 15% ID/g and similar to 20% ID/g at 8 h p.i. with minor losses after 6 days. [Zr-89]Zr-citrate bone uptake was also observed, but [Zr-89]Zr-phosphate was absorbed in high amounts in the liver and the spleen. The marrow cells were insignificantly radioactive in comparison to the calcified tissues.
Conclusion: Despite the complexity of Zr coordination, the electrophoretic
analyses provided detailed evidences of Zr charges either as salts or as complexes. This study also shows that weakly chelated, Zr-89 is a bone seeker and has a strong affinity for phosphate. (C) 2011 Elsevier Inc. All rights reserved.”
“Human adenoviruses (HAdV) AZD9291 manufacturer and JC polyomaviruses (JCPyV) have been proposed as markers of fecal/urine contamination of human origin. An indirect immunofluorescence assay has been developed to quantify infectious human adenoviruses types 2 and 41 and JC polyomaviruses strain Mad-4 in water samples. The immunofluorescence assay was compared with other quantitative techniques used commonly such as plaque assay, tissue culture infectious dose-50 and quantitative PCR (qPCR). The immunofluorescence assays showed to be specific for the detection of infectious viruses, obtaining negative results when UV or heat-inactivated viruses were analyzed.