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“The env open reading frames of African lion (Panthera leo) lentivirus (feline immunodeficiency virus [FIVPle]) subtypes B and E from geographically distinct regions of Africa suggest two distinct ancestries, with FIVPle-E sharing a common ancestor with the domestic cat (Felis catus) lentivirus (FIVFca). Here we demonstrate that FIVPle-E and FIVFca share the use of CD134 (OX40) and CXCR4 as a primary receptor

and coreceptor, respectively, and that both lion CD134 and CXCR4 are functional receptors for FIVPle-E. The shared usage of CD134 and CXCR4 by see more FIVFca and FIVPle-E may have implications for in vivo cell tropism and the pathogenicity of the E subtype among free-ranging lion populations.”
“Introduction: Bromine-76-radiolabeled analogues of previously reported high-affinity A(3) adenosine receptor (A(3)AR) nucleoside ligands have been prepared as potential radiotracers for positron emission tomography.

Methods: The radiosyntheses were accomplished by oxidative radiobromination find more on the N-6-benzyl moiety of trimethyltin precursors. Biodistribution studies of the kinetics Of uptake were conducted in awake rats.

Results: We prepared an agonist ligand [Br-76](1′S,2′R,3′S,4′R,5′S)-4′-2-chloro-6-[(3-bromophenylmethyl)amino]purin-9-yl-1′-(methylaminocarbonyl)bicyclo[3.1.0]hexane-2′,3′-diol

(MRS3581) in 59% radiochemical yield with a specific activity of 19.5 GBq/mu mol and all antagonist ligand {[Br-76](1′R,2′R,3′S,4′R,5′S)-4′-(6-(3-bromobenzylamino)-2-clhloro-9H-purin-9-yl)bicyclo[3.1.0]hexane-2′,3′-diol (MRS5147) in 65% radiochemical yield with a specific activity of 22 GBq/mu mol. The resultant products exhibited the expected high affinity (K-i similar to 0.6 nM) and specific binding at the human A(3)AR in vitro. Biodistribution studies in the rat showed uptake in the organs of excretion and metabolism. The antagonist MRS5147 exhibited increasing uptake

in testes, an organ that contains significant quantities of A(3)AR, over a 2-h time course, which suggests the presence of a specific A(3)AR retention mechanism.

Conclusion: We were able to compare uptake of the [Br-76]-labeled antagonist MRS5147 to [Br-76]agonist MRS3581. The antagonist MRS5147 shows increasing uptake in the testes, an A(3)AR-rich tissue, suggesting that Oxygenase this ligand may have promise as a molecular imaging agent. Published by Elsevier Inc.”
“The transmission of variant Creutzfeldt-Jakob disease (vCJD) through blood transfusions has created new concerns about the iatrogenic spread of transmissible spongiform encephalopathies (TSEs)/prion diseases through blood and plasma-derived products and has increased the need to develop efficient methods for detection of the agent in biologics. Here, we report the first successful generation of spleen-derived murine stromal cell cultures that persistently propagate two mouse-adapted isolates of human TSE agents, mouse-adapted vCJD, and Fukuoka 1.