Furthermore, it was reported that YKS inhibited skin lesions in socially isolated mice but not in group-housed mice. Therefore, in the present study it was investigated whether or not YKS was effective in the treatment of AD using socially isolated NC/Nga mice.
Objective: The present study was designed to assess the effect of YKS on the development of AD-like lesions in socially isolated NC/Nga mice to obtain information about its usefulness in the treatment of AD.
Methods:
Ten-week-old male NC/Nga mice were socially isolated under conventional conditions. YKS was administered orally to mice at the dose of 0.5% or 1.0% together with diet. The efficacy GW786034 chemical structure of YKS was evaluated by assessing skin lesion severity, scratching behaviors, skin hydration, and infiltration of inflammatory cells in the skin. Grooming behaviors evoked by social isolation PLX4032 inhibitor stress and serum corticosterone levels were also measured.
Results: Oral administration of YKS to socially isolated
NC/Nga mice resulted in the inhibition of exacerbation of AD-like skin lesions. It seemed that the inhibition of exacerbation of AD-like skin lesions observed in NC/Nga mice might be due to suppression of the scratching and grooming behaviors, inhibition of the infiltration of mast cells and eosinophils, and retention of humidity in the skin. Serum corticosterone levels were also significantly inhibited in the 1%-YKS-treated mice as compared with those of the control mice. There were no significant differences in the levels of serum total IgE and nerve growth factor (NGF) between the YKS-treated mice and the non-treated control mice.
Conclusion: YKS inhibited the development of AD-like skin lesions in socially isolated NC/Nga mice by suppressing scratching Vorinostat Epigenetics inhibitor and infiltration of inflammatory cells in the skin. These results indicate
that YKS possesses an anti-itching property, and its anti-itching may be partly through attenuation on social isolation stress. It is expected that YKS might provide an effective alternative therapy for AD in human patients. (C) 2009 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.”
“Objective-To evaluate the use of a ketamine-propofol combination, with or without dexmedetomidine, in cats undergoing ovariectomy and to assess Heinz body formation following administration of these drugs.
Design-Randomized clinical trial.
Animals-15 client-owned female cats.
Procedures-Anesthesia was induced with a ketamine (2.0 mg/kg [0.91 mg/lb])-propofol (2.0 mg/kg) combination with (n = 7) or without (8) dexmedetomidine (0.003 mg/kg [0.0013 mg/lb]) and was maintained via continuous IV infusion of a 1:1 ketamine-propofol combination (administration rate for each drug, 10.0 mg/kg/h [4.54 mg/lb/h]). Cats underwent ovariectomy; duration of infusion was 25 minutes. Physiologic variables were measured at predetermined time points. Heinz bodies were quantified via examination of blood smears.