Addition of glycerol significantly affects WVP and P′O2 (P < 0.05). Since the main function of a food packaging

is often to avoid or at least to decrease moisture transfer between the food and the surrounding atmosphere, WVP should be as low as possible ( Mali et al., 2006). The regression analysis, using response surface methodology, was applied on results of WVP and P′O2 of the films indicating that both components glycerol (G) and clay nanoparticles (C) influenced significantly WVP and P′O2, however only for WVP, expressed by Equation (5), in real values, was obtained buy Enzalutamide with good correlation (r2 = 77%). As can be observed in Fig. 2(b), biodegradable films produced with lower contents of glycerol and higher contents of clay nanoparticles presented lower WVP. equation(5) WVP=(2.65+3.77×G−19.5×C)±0.71(0.75≤G≤1.25)(0.00≤C≤0.10)wherein WVP is the water vapor permeability [g mm m−2 d−1 kPa−1]; G is the glycerol content [g/100 g of filmogenic solution]; and C is the clay nanoparticles content [g/100 g of filmogenic solution]. In

order to compare these results with those of classic materials, cellophane water vapor permeability was obtained with assays using the same conditions of the tests performed with BF and the result ((0.49 ± 0.02) g mm m−2 d−1 kPa−1) selleck chemicals llc was 10 times lower than for the BF. Comparable results of WVP were shown by commercial materials produced by Cargill Dow (USA) under the Natureworks® trade mark and by Solvay (Belgium) under the CAPA® trade mark ( Avérous, 2004). Glass transition temperatures obtained from DSC experiments are reported in Table 3. The results showed the same behavior for all samples of BF elaborated, independent of glycerol and clay contents. Two distinct

glass transition temperatures, associated with two heat capacity changes in the samples, were observed in all formulations produced, the first varying from (35 to 39) °C and the second one from (53 to 63) °C. Similar values were observed in other polymeric materials. Polylactic acid (PLA), a biodegradable polyester commonly used for trays, cups, bottles and films, has been industrially not produced by Cargill Dow (USA) under the Natureworks® trade mark, with a similar glass transition temperature: 58 °C (Avérous, 2004). Tang et al. (2008) fabricated biodegradable nanocomposites from corn starch and montmorillonite nanoclays by melt extrusion processing, with Tg varying from (50.71 ± 2.76) °C to (54.74 ± 1.21) °C, when water content of starch-clay nanocomposite decreased from (13.06 ± 1.73) g/100 g to (9.75 ± 0.21) g/100 g. Specimens fabricated by injection molding using pellets produced with wheat starch (74 g/100 g), glycerol (10 g/100 g) and water (16 g/100 g) presented Tg of 43 °C ( Avérous, Fauconnier, Moro, & Fringant, 2000). Arvanitoyannis, Psomiadou, and Nakayama (1996) observed a decrease on glass transition temperature of edible films based on corn starch and plasticized with glycerol from (88.8 ± 3.4) °C to (33.0 ± 1.