A GP profile could be a great contribution to construct pathways of lipid biosynthesis and metabolism. Consequently, the results show that profiling methods are important for further elucidation of the manifold roles of lipids. To our knowledge,
this work also presents, for the first time, a comprehensive Inhibitors,research,lifescience,medical GP profile of yeast strains other than the widely studied S. cerevisiae, some of which are appreciated for biotechnological or basic research purposes. The results could be an important first step for further elucidation of the roles of lipids in these particular strains, which would contribute BMS-754807 order significantly to new scientific perceptions. Acknowledgments We acknowledge Helma Geltenpoth for the skilled technical assistance in glycerophospholipid extraction, Rita Inhibitors,research,lifescience,medical Fobbe and Jürgen Nolte for fatty acid analysis by GC/MS and Lars M. Blank for yeast expertise. Financial support by the Ministerium für Innovation, Wissenschaft, Forschung und Technologie des Landes Nordrhein-Westfalen‘ (Düsseldorf, Germany) and by the ‚Bundesministerium für Bildung und Forschung‘ (Bonn, Germany) is gratefully acknowledged. Supplementary Files Supplementary
File 1 XLS-Document (XLS, 83 KB) Click here for additional data file.(83K, Inhibitors,research,lifescience,medical xls) Conflict of Interest Conflict of Interest The authors declare no conflict Inhibitors,research,lifescience,medical of interest.
The bacterial genus Streptomyces is well known for the production of numerous clinically used antibiotics. These filamentous soil bacteria undergo a complex developmental cycle, and antibiotic production usually occurs as part of the secondary metabolism of non-growing stationary cultures. The antibiotic biosynthetic pathway enzymes are induced Inhibitors,research,lifescience,medical while cell growth ceases during the transition from growth to secondary metabolism . The molecular understanding of this cellular reorganization
taking place during this transition phase is of importance for improving the strain’s antibiotic producing capabilities. Several studies focusing on gene expression have been undertaken to reveal more about this complex metabolic switch [2,3]. However, analyses at the protein, metabolite and metabolic fluxes level are also needed however to gain a more complete picture and understanding of cellular behavior and properties. In this regard, systems biology has emerged as a concept for integrating global experimental datasets covering several levels of metabolism using statistical tools and mathematical modeling . S. coelicolor is the most-studied streptomycete, and sequencing of the genome revealed the presence of more than 20 cryptic gene clusters for secondary metabolites in addition to the well known secondary metabolites actinorhodin and undecylprodigiosin . S.